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KMID : 0379119890170010027
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Korean Journal of Mycology
1989 Volume.17 No. 1 p.27 ~ p.30
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Transformation of the β-Isopropylmalate Dehydrogenase Gene of Flammulina velutipes into Pleurotus florida
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박용환/Park, Yong Hwan
유영복/유창현/변명옥/고승규/차동열/Yoo, Young Bok/You, Chang Hyun/Byun, Myung Ok/Go, Seung Joo/Cha, Dong Yul
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Abstract
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F. velutipes Leu 2 gene (β-isopropylmalate dehydrogenase gene) was used for transformation of P. florida leucine requiring auxotrophic mutant P101. Transformation frequency was very low but the transformed colony can grow on minimal medium very slowly. Transformation was identified by Southern hybridization and reverse transformation into E. coli using chromosome DNA isolated from transformed P. florida.
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